The Pinckney Island National Wildlife Refuge is a 4,053-acre (16 km2) National Wildlife Refuge located in Beaufort County, South Carolina between the mainland and Hilton Head Island. Named after Major General Charles Cotesworth Pinckney, it was established to provide a nature and forest preserve for aesthetic and conservation purposes.

Beaufort County is a county in the U.S. state of South Carolina. As of the 2020 census, its population was 187,117. Its county seat is Beaufort and its largest community is Hilton Head Island.