Port Washington is the terminus of the Long Island Rail Road's Port Washington Branch in Port Washington, New York. The station is located on Main Street, between Haven Avenue and South Bayles Avenue, just west of Port Washington Boulevard, and is 19.9 miles (32 km) from Pennsylvania Station in Midtown Manhattan. A pedestrian bridge exists between the platforms, and is in line with Franklin Avenue, ending at Haven Avenue.

The Long Island Rail Road, often abbreviated as the LIRR, is a railroad in the southeastern part of the U.S. state of New York, stretching from Manhattan to the eastern tip of Suffolk County on Long Island. The railroad currently operates a public commuter rail service, with its freight operations contracted to the New York and Atlantic Railway. With an average weekday ridership of 354,800 passengers in 2016, it is the busiest commuter railroad in North America. It is also one of the world's few commuter systems that runs 24/7 year-round. It is publicly owned by the Metropolitan Transportation Authority, which refers to it as MTA Long Island Rail Road. In 2023, the system had a ridership of 75,186,900, or about 253,800 per weekday as of the fourth quarter of 2023.