The pre-Columbian cultures of Colombia refers to the ancient cultures and civilizations that inhabited Colombia before the Spanish Conquest in the 16th century.

The Muisca are an indigenous people and culture of the Altiplano Cundiboyacense, Colombia, that formed the Muisca Confederation before the Spanish conquest. The people spoke Muysccubun, a language of the Chibchan language family, also called Muysca and Mosca. They were encountered by conquistadors dispatched by the Spanish Empire in 1537 at the time of the conquest. Subgroupings of the Muisca were mostly identified by their allegiances to three great rulers: the hoa, centered in Hunza, ruling a territory roughly covering modern southern and northeastern Boyacá and southern Santander; the psihipqua, centered in Muyquytá and encompassing most of modern Cundinamarca, the western Llanos; and the iraca, religious ruler of Suamox and modern northeastern Boyacá and southwestern Santander.