Preston Lions Football Club is a semi-professional soccer club from Preston, a northern suburb of Melbourne, Victoria, Australia. The club was established in 1947 to play non-professional soccer matches against other teams in the local community until 1959, and 12 years later, they joined the Victorian Soccer Federation, the club's first season in an officially sanctioned competition. The Lions currently compete in the Victoria Premier League 1 competition, which is the third tier in the Australian soccer league system, after being promoted in 2022 from NPL 3 Victoria. The club won the Victorian Premier League in seasons 1980, 1994, 2002 and 2007.

Preston is a suburb in Melbourne, Victoria, Australia, 9 km (5.6 mi) north-east of Melbourne's central business district, located within the City of Darebin local government area. Preston recorded a population of 33,790 at the 2021 census.