Project Strato-Lab was a high-altitude manned balloon program sponsored by the United States Navy during the 1950s and early 1960s. The Strato-Lab program lifted the first Americans into the upper reaches of the stratosphere since World War II. Project Strato-Lab developed out of the Navy's unmanned balloon program, Project Skyhook. The program was established in 1954 and administrated by Commander Malcolm Ross. Malcolm Ross and others developed the program to accomplish research required for the manned rocket program to follow. This program provided biomedical data that was used for subsequent efforts in space. Malcolm Ross launched five numbered flights as well as other unnumbered flights.

Lieutenant Commander Victor Alonzo Prather Jr. was an American flight surgeon famous for taking part in "Project RAM", a government project to develop the space suit. On May 4, 1961, Prather drowned during the helicopter transfer after the landing of the Strato-Lab V balloon flight, which set an altitude record for manned balloon flight which stood until 2012.