Protein mass spectrometry
Videos
Protein mass spectrometry refers to the application of mass spectrometry to the study of proteins. Mass spectrometry is an important method for the accurate mass determination and characterization of
A mass spectrometer used for high throughput protein analysis.
Chromatography trace and MS/MS spectra of a peptide.
Electrospray ionization
Videos
Electrospray ionization is a technique used in mass spectrometry to produce ions using an electrospray in which a high voltage is applied to a liquid to create an aerosol. It is especially useful in p
Protein mass spectrometry
…between different proteins as well as with membrane lipids. The two primary methods used for the ionization of protein in mass spectrometry are electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI). These ionization techniques are used in conjunction with mass analyzers such as tandem…
Electrospray (nanoSpray) ionization source
Diagram of electrospray ionization in positive mode: under high voltage, the Taylor cone emits a jet of liquid drops. The solvent from the droplets progressively evaporates, leaving them more and more charged. When the charge exceeds the Rayleigh limit the droplet explosively dissociates, leaving a stream of charged (positive) ions
Fenn's first electrospray ionization source coupled to a single quadrupole mass spectrometer
Diagram of a DESI ambient ionization source