Protein sequencing
Videos
Protein sequencing is the practical process of determining the amino acid sequence of all or part of a protein or peptide. This may serve to identify the protein or characterize its post-translational
A Beckman-Coulter Porton LF3000G protein sequencing machine
Using a Beckman-Spinco Protein-Peptide Sequencer, 1970
SDS-PAGE
Videos
SDS-PAGE is a discontinuous electrophoretic system developed by Ulrich K. Laemmli which is commonly used as a method to separate proteins with molecular masses between 5 and 250 kDa. The combined use
Proteins of the erythrocyte membrane separated by SDS-PAGE according to their molecular masses
Sample combs with different numbers of pockets, each prong leaves a pocket in the gel when pulled out
Polymerised separating and stacking gel before removing the sample comb (white) between the spacers (black), in the stacking gel are small amounts of bromophenol blue for improved visibility, the separating gel is unstained
Electrophoresis chamber after a few minutes of electrophoresis. In the first pocket a size marker was applied with bromophenol blue, in the other pockets, the samples were added bromocresol green