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Pseudouridine
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Pseudouridine is an isomer of the nucleoside uridine in which the uracil is attached via a carbon-carbon instead of a nitrogen-carbon glycosidic bond.
Pseudouridine is biosynthesized from uridine via the action of Ψ synthases.
Pseudouridine is biosynthesized from uridine via the action of Ψ synthases.
Pseudouridine synthase bound with tRNA.
Pseudouridine synthase bound with tRNA.
Spliceosome
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A spliceosome is a large ribonucleoprotein complex found primarily within the nucleus of eukaryotic cells. The spliceosome is assembled from small nuclear RNAs and numerous proteins. Small nuclear RNA
Spliceosomal splicing cycle
Spliceosomal splicing cycle
Figure 1. Above are electron microscopy fields of negatively stained yeast (Saccharomyces cerevisiae) tri-snRNPs. Below left is a schematic illustrati
Figure 1. Above are electron microscopy fields of negatively stained yeast (Saccharomyces cerevisiae) tri-snRNPs. Below left is a schematic illustration of the interaction of tri-snRNP proteins with the U4/U6 snRNA duplex. Below right is a cartoon model of the yeast tri-snRNP with shaded areas corresponding to U5 (gray), U4/U6 (orange) and the linker region (yellow).