Puerto Ayora is a town in central Galápagos, Ecuador. Located on the southern shore of Santa Cruz Island, it is the seat of Santa Cruz Canton. The town is named in honor of Isidro Ayora, an Ecuadorian president. The town is sometimes mistakenly referred to as Santa Cruz. Puerto Ayora is the most populous town in the Galápagos Islands, with a population of 12,696.

Galápagos is a province of Ecuador in the country's Insular region, located approximately 1,000 km (620 mi) off the western coast of the mainland. The capital is Puerto Baquerizo Moreno.