Puerto Berrío is a municipality and town in the Colombian department of Antioquia. It is part of the Magdalena Medio Antioquia sub-region.

The Magdalena River is the main river of Colombia, flowing northward about 1,528 kilometres (949 mi) through the western half of the country. It takes its name from the biblical figure Mary Magdalene. It is navigable through much of its lower reaches, in spite of the shifting sand bars at the mouth of its delta, as far as Honda, at the downstream base of its rapids. It flows through the Magdalena River Valley.