Punisher Kills the Marvel Universe is a 1995 one-shot comic book published by Marvel Comics. Written by Garth Ennis and illustrated by Doug Braithwaite with most inking done by Michael L. Halblieb, the story depicts Frank Castle killing every superhero and supervillain in the Marvel Universe after his family is killed.

Garth Ennis is a Northern Irish–American comics writer, best known for the Vertigo series Preacher with artist Steve Dillon, his nine-year run on Marvel Comics' Punisher franchise, and The Boys with artist Darick Robertson. He has collaborated with artists such as Dillon and Glenn Fabry on Preacher, John McCrea on Hitman, Marc Silvestri on The Darkness, and Carlos Ezquerra on both Preacher and Hitman. His work has won him recognition in the comics industry, including nominations for the Comics Buyer's Guide Award for Favorite Writer in 1997, 1998, 1999, and 2000.