The Q27 bus route constitutes a public transit line in Queens, New York City, running primarily along 46th Avenue, Rocky Hill Road and Springfield Boulevard between a major bus-subway hub in Flushing and Cambria Heights. The route is operated by MTA Regional Bus Operations under the New York City Transit brand.

Cambria Heights is a residential neighborhood in the southeastern portion of the New York City borough of Queens. It is bounded by Springfield Boulevard and Francis Lewis Boulevard to the west, the Elmont, Nassau County border on the east, Queens Village to the north, St. Albans to the west, and Montefiore Cemetery and Laurelton, Springfield Gardens, and Rosedale to the south. As of 2010, Cambria Heights's population was 18,677. The neighborhood is part of Queens Community Board 13.