Queen Street Mill is a former weaving mill in Harle Syke, a suburb to the north-east of Burnley, Lancashire, that is a Grade I listed building. It now operates as a museum and cafe. Currently open for public tours between April and November. Over winter the café is opened on Wednesdays. It is also viewable with private bookings.

Harle Syke is a small village within the parish of Briercliffe, situated three miles north of Burnley, Lancashire, England. It was the home to eleven weaving firms, working out of seven mills. Queen Street Mill closed in 1982, and was converted to a textile museum, preserving it as a working mill. It is the world's last 19th-century steam powered weaving mill.