Queens Quay is an underground streetcar station of the Toronto streetcar system in Toronto, Ontario, Canada. Along with Spadina station and Union station, it is one of three stations open overnight to support late-night streetcar routes. It is the only underground streetcar station that is not part of or connected to a Toronto subway station. It was opened in 1990 as part of the former Harbourfront LRT route. The station is now served by the 509 Harbourfront, 510 Spadina daytime routes and the 310 Spadina night route.

The Toronto streetcar system is a network of eleven streetcar routes in Toronto, Ontario, Canada, operated by the Toronto Transit Commission (TTC). It is the third busiest light-rail system in North America. The network is concentrated primarily in Downtown Toronto and in proximity to the city's waterfront. Much of the streetcar route network dates from the 19th century. Three streetcar routes operate in their own right-of-way, one in a partial right-of-way, and six operate on street trackage shared with vehicular traffic with streetcars stopping on demand at frequent stops like buses. Since 2019, the network has used low-floor streetcars, making it fully accessible.