The R27 is a provincial route in South Africa that consists of two disjoint segments. The first segment, also known as the West Coast Highway, connects Cape Town with Velddrif along the West Coast. The second runs from Vredendal via Vanrhynsdorp, Calvinia, Brandvlei and Kenhardt to Keimoes on the N14 near Upington. The connection between Velddrif and Vredendal has never been built, although it can be driven on various gravel roads.

Provincial routes are the second category of road in the South African route-numbering scheme. They are designated with the letter "R" followed by a number from 21 to 82, formerly with the letter "P" followed by a number from 66. They serve as feeders to the national routes and as trunk roads in areas where there is no national route.