RPMI 1640
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RPMI 1640, simply known as RPMI medium, is a cell culture medium commonly used to culture mammalian cells. RPMI 1640 was developed by George E. Moore, Robert E. Gerner, and H. Addison Franklin in 1966
Tissue culture flasks
Hybridoma technology
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Hybridoma technology is a method for producing large quantities of monoclonal antibodies by fusing antibody producing B cells with myeloma cells. This creates hybrid cells, hybridomas, that produce th
RPMI 1640
…serum replacement, RPMI 1640 allows the cultivation of many cell types, especially human lymphocytes, Jurkat cells, HeLa cells, bone marrow cells, hybridomas and carcinomas. Many different formulations exist. Typically, one liter of RPMI 1640 contains…
Hybridoma cells grown in tissue culture. The image shows a single clone of cells each of which is producing large amounts of a specific monoclonal antibody which the cells secrete and which can be readily purified from the culture media.