Rail transport in the Australian state of Victoria is provided by a number of railway operators who operate over the government-owned railway lines. The network consists of 2,357 km of Victorian broad gauge lines, and 1,912 km of standard gauge freight and interstate lines; the latter increasing with gauge conversion of the former. Historically, a few experimental 762 mm gauge lines were built, along with various private logging, mining and industrial railways. The rail network radiates from the state capital, Melbourne, with main interstate links to Sydney and to Adelaide, as well as major lines running to regional centres, upgraded as part of the Regional Fast Rail project.

Rail gauges in Australia display significant variations, which has presented an extremely difficult problem for rail transport on the Australian continent since the 19th century. As of 2022, there are 11,914 kilometres (7,403 mi) of narrow-gauge railways, 18,007 kilometres (11,189 mi) of standard gauge railways and 2,685 kilometres (1,668 mi) of broad gauge railways. In the 19th century, each of the colonies of Australia adopted their own gauges.