The Randolph family of Virginia is a prominent political family, whose members contributed to the politics of Colonial Virginia and Virginia after statehood. They are descended from the Randolphs of Morton Morrell, Warwickshire, England. The first Randolph in America was Edward Fitz Randolph, who settled in Massachusetts in 1630. His nephew, William Randolph, later came to Virginia as an orphan in 1669. He made his home at Turkey Island along the James River. Because of their numerous progeny, William Randolph and his wife, Mary Isham Randolph, have been referred to as "the Adam and Eve of Virginia". The Randolph family was the wealthiest and most powerful family in 18th-century Virginia.

William Randolph I was an English-born planter, merchant and politician in colonial Virginia who played an important role in the development of the colony. Born in Moreton Morrell, Warwickshire, Randolph moved to the colony of Virginia sometime between 1669 and 1673, and married Mary Isham a few years later. His descendants include many prominent individuals including Thomas Jefferson, John Marshall, Paschal Beverly Randolph, Robert E. Lee, Peyton Randolph, Edmund Randolph, John Randolph of Roanoke, George W. Randolph, and Edmund Ruffin. Due to his and Mary's many progeny and marital alliances, they have been referred to as "the Adam and Eve of Virginia".