Rattlesnake Hills is an American Viticultural Area (AVA) located in Yakima County, Washington surrounding the Rattlesnake Hills. The appellation is the state's 9th AVA established on March 20, 2006 by the Alcohol and Tobacco Tax and Trade Bureau {TTB), Treasury after reviewing the petition submitted by Mr. Gail Puryear, Bonair Winery owner, along with 10 other vineyard and winery owners in the region proposing the "Rattlesnake Hills" viticultural area. The 68,500 acres (107 sq mi) Rattlesnake Hills AVA boundaries are within the Yakima Valley AVA as both are encompassed by the vast Columbia Valley AVA. Rattlesnake Hills borders the northern boundary of Yakima Valley including land between the north bank of the Sunnyside Canal and the entire southern boundary slopes are between Outlook and the Wapato Dam. The western boundary meanders on an east-west chain of hills north of I-82 passing through the cities of Parker, Donald, Buena and Zillah while just east of the Yakima River. Rattlesnake Hills rises to the highest point in the Yakima Valley with elevations ranging from 850 to 3,085 feet (259–940 m).

Yakima County is a county in the U.S. state of Washington. As of the 2020 census, its population was 256,728. The county seat and most populous city is Yakima. The county was formed out of Ferguson County in January 1865 and is named for the Yakama tribe of Native Americans.