The Regional Assembly of Murcia is the autonomous parliament of the Region of Murcia, one of the autonomous communities of Spain. The unicameral assembly, which contained 45 elected legislative seats, is located in the Murcian city of Cartagena, Spain.

The Region of Murcia is an autonomous community of Spain located in the southeastern part of the Iberian Peninsula, on the Mediterranean coast. The region is 11,313 km2 (4,368 sq mi) in area and had a population of 1,511,251 as at the start of 2020. About a third of its population lives in the capital, Murcia, and a seventh in the second city, Cartagena. At 2,014 m (6,608 ft), the region's highest point is Los Obispos Peak in the Massif of Revolcadores.