The Regional Assembly of Murcia is the autonomous parliament of the Region of Murcia, one of the autonomous communities of Spain. The unicameral assembly, which contained 45 elected legislative seats, is located in the Murcian city of Cartagena, Spain.

Cartagena is a Spanish city and a major naval station on the Mediterranean coast, south-eastern Iberia. As of January 2018, it has a population of 218,943 inhabitants. This makes Cartagena Murcia's second-largest municipality and Spain's sixth-largest city that is not a provincial-capital. The wider urban or metropolitan area of Cartagena, known as Campo de Cartagena, has a population of 409,586 inhabitants.