Renée of France, was Duchess of Ferrara from 31 October 1534 until 3 October 1559 by marriage to Ercole II d'Este, grandson of Pope Alexander VI. She was the younger surviving child of Louis XII of France and the duchess regnant Anne of Brittany. In her later life, she became an important supporter of the Protestant Reformation and ally of John Calvin.

Ercole II d'Este was Duke of Ferrara, Modena and Reggio from 1534 to 1559. He was the eldest son of Alfonso I d'Este and Lucrezia Borgia.