Restriction enzyme
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A restriction enzyme, restriction endonuclease, REase, ENase or restrictase is an enzyme that cleaves DNA into fragments at or near specific recognition sites within molecules known as restriction sit
Different restriction enzymes acting on different recognition sites produce different DNA fragments
Molecular cloning
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Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word c
Diagram of a commonly used cloning plasmid; pBR322. It's a circular piece of DNA 4361 bases long. Two antibiotic resistance genes are present, conferring resistance to ampicillin and tetracycline, and an origin of replication that the host uses to replicate the DNA.