Rio is a village in Columbia County, Wisconsin, United States. The population was 1,133 at the 2022 United States Census. It is part of the Madison Metropolitan Statistical Area. The village rests primarily in the town of Otsego. The Village of Rio calls itself "Camper Country USA" due to several campgrounds located near the village. Rio is halfway between Columbus and Portage on Highway 16.

Columbia County is a county located in the U.S. state of Wisconsin. As of the 2020 census, the population was 58,490. Its county seat and largest city is Portage. The county was created in 1846 as part of Wisconsin Territory.