The River Lathkill is a river in the Peak District National Park in Derbyshire, England.

The Peak District is an upland area in England, at the southern end of the Pennines. Mostly in Derbyshire, it extends into Cheshire, Greater Manchester, Staffordshire, West Yorkshire and South Yorkshire. It is subdivided into the Dark Peak, moorland dominated by gritstone, and the White Peak, a limestone area with valleys and gorges. The Dark Peak forms an arc on the north, east and west of the district, and the White Peak covers central and southern areas. The highest point is Kinder Scout. Most of the area is within the Peak District National Park, a protected landscape designated in 1951.