The Diocese of Prato is a Latin diocese of the Catholic Church in Tuscany, that has existed as an independent entity since 1954. Its see is Prato Cathedral, some 10 miles (17 km) northwest of Florence, on the Bisenzio River. The diocese is a suffragan of the Archdiocese of Florence.

Prato Cathedral, or Cathedral of Saint Stephen, is a Roman Catholic cathedral in Prato, Tuscany, Central Italy, from 1954 the seat of the Bishop of Prato, having been previously, from 1653, a cathedral in the Diocese of Pistoia and Prato. It is dedicated to Saint Stephen, the first Christian martyr.