The Roman Ghetto or Ghetto of Rome was a Jewish ghetto established in 1555 in the Rione Sant'Angelo, in Rome, Italy, in the area surrounded by present-day Via del Portico d'Ottavia, Lungotevere dei Cenci, Via del Progresso and Via di Santa Maria del Pianto, close to the River Tiber and the Theatre of Marcellus. With the exception of brief periods under Napoleon from 1808 to 1815 and under the Roman Republics of 1798–99 and 1849, the ghetto of Rome was controlled by the papacy until the capture of Rome in 1870.

In the early modern era, European Jews were confined to ghettos and placed under strict regulations as well as restrictions in many European cities. The character of ghettos fluctuated over the centuries. In some cases, they comprised a Jewish quarter, the area of a city traditionally inhabited by Jews. In many instances, ghettos were places of terrible poverty and during periods of population growth, ghettos had narrow streets and small, crowded houses. Residents had their own justice system. Around the ghetto stood walls that, during pogroms, were closed from inside to protect the community, but from the outside during Christmas, Pesach, and Easter Week to prevent the Jews from leaving at those times.