Colonel Rosa Klebb is a fictional character, the main antagonist in the James Bond 1957 novel and 1963 film From Russia with Love, in which she is played by Lotte Lenya. She was a Soviet counter-intelligence operative until being discharged and joining SPECTRE.

From Russia, with Love is the fifth novel by the English author Ian Fleming to feature his fictional British Secret Service agent James Bond. Fleming wrote the story in early 1956 at his Goldeneye estate in Jamaica; at the time he thought it might be his final Bond book. The novel was first published in the United Kingdom by Jonathan Cape on 8 April 1957.