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Rosemary Prinz

Rosemary Prinz is a stage and television actress. She is most known for her work in the early era of the American soap opera. Prinz originated the role of M'Lynn Eatenton in Steel Magnolias during its first production Off-Broadway. Prinz was born in Manhattan, her father, Milton Prinz, was a talented cellist and Prinz herself spent her early years in the theater. After graduating from high school at age sixteen, she made her summer stock debut in a 1947 production of Dream Girl. In 1952 she made her Broadway debut as a girl scout in The Grey-Eyed People and returned to Broadway in 1978 for a production of Tribute with Jack Lemmon. Prinz has continued to work in all forms of theater, including in recent years, Cat on a Hot Tin Roof, Master Class and Annie Get Your Gun, a 2003 New York appearance in Killing Louise. Prinz made her television debut in the short-lived 1954 daytime drama First Love, as the wife of aviator Chris, her most famous role to date has been her portrayal of Penny Hughes on As the World Turns, a role she played from 1956-68.

Penny had a number of storylines, but her most popular story was her tortured relationship with Jeff Baker. They were daytime's first teen romance and one of the show's most popular couples, breaking up and reuniting many times; the couple happily married and planned to adopt a child. Their story peaked when Jeff was killed in a car crash and Penny suffered from amnesia. Viewers were outraged. Over the years, rumors surfaced that Prinz had been pushed to many nervous breakdowns due to the constant criticisms that she endured from show creator Irna Phillips; when she left the show in 1968, Prinz said. However, she was lured back, but only for a limited engagement each time, her first return was to play the role of Amy Tyler on All My Children for six months in 1970. Prinz agreed, on the condition that her character oppose the Vietnam War, which Prinz herself opposed, on the condition that she be given above-the-title billing. Prinz was the only All My Children performer to receive that honor. Prinz was until 1990, the only All My Children actor to have her photo in the opening credits.

This role was followed by a nine-month turn as the lead character, Dr. Julie Franklin, in How to Survive a Marriage in 1974. In 1988, she became the last actress to play Sister Mary Joel on Ryan's Hope, she made several returns during events that focused on Penny's family. She returned in 1985 for Bob and Kim's wedding and in 1986 to celebrate her parents' fiftieth wedding anniversary, she returned to As the World Turns again in 1998 so Penny could attend her mother's eightieth birthday party, in 2000 to visit her family for Christmas. However, Prinz were not part of the show's finale in 2010, or part of the funeral for show matriarch Nancy Hughes, Penny's mother. Prinz starred in the national tour of The Apple Tree in 1967, her co-stars were Will MacKenzie. She starred in the second national tour of Driving Miss Daisy. In the late 1970s, Prinz began to make rare prime-time television appearances, including a recurring role on Knots Landing from 1981–1982 as Sylvia Warren, convinced her husband was having an affair with Laura Avery.

She appeared in the 1980 Hart to Cruise at Your Own Risk, as Esther Goldwin. Another prime time role was on Shirley. In 2000 at the age of seventy, Prinz made her movie debut in the low-budget The Bread, My Sweet, which starred Scott Baio; until that time the closest she had come to moviemaking was a 1948 film for the Navy, It Could Happen to Your Sister, in which she played a young woman who contracted an STD. In 2004 she completed Extreme Mom. Prinz was married to actor Michael Thoma from 1951–57, has been married to jazz drummer Joseph Patti since 1967. 2003 interview Rosemary Prinz on IMDb

Next Ukrainian parliamentary election

According to the electoral Code of Ukraine the next Ukrainian parliamentary election will be held on the last Sunday of October of the fifth year of authority of the parliament. The last parliamentary elections in Ukraine were held on 21 July 2019. On 1 January 2020 the latest reversion of the electoral code of Ukraine took effect, it states that deputies can only be elected on a party list in one nationwide constituency with a 5% election threshold with open regional lists of candidates for deputies. The new election law abolish the single-member constituencies system used in the 2019 parliamentary election. In these elections the 450 members of the Verkhovna Rada were elected by two methods. On 4 February 2020 parliament approved a Presidential bill to cut down the number of parliamentary deputies from 450 to 300. To do so the Ukrainian Constitution needs to be altered, this will require at least 300 parliamentary votes. Central Election Commission of Ukraine

Hennion two-seater

The Hennion was a two seat French training and touring aircraft, completed just before World War II began. It was flown again post-war and fitted with a new engine. Emile Hennion was the chief pilot at the Casablanca Aero Club in the 1930s. Just before the outbreak of World War II he designed and flew a side-by-side seat two seat aircraft, powered by a Train 4A 01 inverted four cylinder air-cooled engine which produced only 30 kW, his goals were safety in the hands of a beginner, economy and true touring ability. The first two features made it of some interest as an ab initio trainer after France had gone to war. In this pre-war form it was known as the Hennion 01, it was a low wing, cantilever monoplane, its wings straight tapered with blunt tips and of quite high aspect ratio. The wings were built around a single spar and fabric covered, though the ailerons were plywood skinned; the fuselage was flat sided and ply skinned, except behind the cockpit where rounded, raised decking was fabric covered.

The Hennion's cockpit was enclosed under a two piece canopy with a sliding rear component. Its two seats were equipped with dual controls. Behind them there were luggage and light baggage/chart spaces 900 mm × 300 mm × 530 mm and 900 mm × 200 mm × 400 mm; the inverted Train engine in the nose drove its fuel tank held 85 l. At the rear the vertical tail was tall, with an unbalanced rudder which reached down to the keel and a fuselage mounted tailplane ahead of it; the Hennion had a tail wheel undercarriage with a track of 2.20 m, its spatted main wheels on vertical, streamlined legs. The Hennion 01 flew for the first time on 28 September 1939 at Casablanca. Despite the low power, it cruised at 125 -- 130 km/h. Cruising, it used about 10 l per hour. Landing speed was 45 km/h; the Hennion 01 survived the war and was flown again in 1949, now on the French civil register as F-WFOY. After a period of disuse, it was re-engined in 1956 with a 48 kW Continental A65 air-cooled flat-four in a revised nose and redesignated the Hennion II.

The extra power gave a useful increase in speed despite an increase in empty weight of about 17%. On 4 February 1962 it was destroyed at Meknes. Hennion 01 pre-war version with Train engine Hennion II 1956 revision with a more powerful Continental engine Data from Gaillard General characteristics Crew: Two Length: 7.30 m Wingspan: 12.60 m Wing area: 15.5 m2 Aspect ratio: 10 Airfoil: NACA 23012 Empty weight: 352 kg Gross weight: 572 kg Powerplant: 1 × Continental A65 air-cooled flat-four, 48 kW Propellers: 2-bladedPerformance Maximum speed: 170 km/h Cruise speed: 145 km/h

SNAP-tag

SNAP-tag is a self-labeling protein tag commercially available in various expression vectors. SNAP-tag is a 182 residues polypeptide that can be fused to any protein of interest and further and covalently tagged with a suitable ligand, such as a fluorescent dye. Since its introduction, SNAP-tag has found numerous applications in biochemistry and for the investigation of the function and localisation of proteins and enzymes in living cells. Compared to the current standard labelling methods used in fluorescence microscopy, the use of SNAP-tag presents significant advantages. Cell biology utilizes tools that allow visualization of proteins in living cells. An important example is the use of fluorescent proteins, such as the green fluorescent protein or yellow fluorescent protein. Molecular biology methods allow these fluorescent proteins to be introduced and expressed in living cells as fusion proteins. However, the photo-physical properties of the fluorescent proteins are not suited for single-molecule spectroscopy.

Fluorescent proteins have, in comparison to commercially available dyes, a much lower fluorescence quantum yield and are destroyed upon excitation with a focused laser beam. The SNAP-tag protein is an engineered version of the ubiquitous mammalian enzyme AGT, encoded in humans by the O-6-methylguanine-DNA methyltransferase gene. SNAP-tag was obtained using a directed evolution strategy, leading to a hAGT variant that accepts O6-benzylguanine derivatives instead of repairing alkylated guanine derivatives in damaged DNA. An orthogonal tag, called CLIP-tag, was further engineered from SNAP-tag to accept O2-benzylcytosine derivatives as substrates, instead of O6-benzylguanine. A split-SNAP-tag version suitable for protein complementation assay and protein-protein interaction studies was developed. Apart from fluorescence microscopy, SNAP-tag and CLIP-tag have proven useful in the elucidation of numerous biological processes, including the identification of multiprotein complexes using various approaches such as FRET, cross-linking, proximity ligation assay.

Other application include the measurement of protein half-lives in vivo, small molecule-protein interactions. Protein tag Halo Tag Darstellung SNAP-Tag und CLIP-Tag Self Labeling Protein Tags. In: Bioforum. Jg. 2005, Nr. 6, S. 50-51

Erik McCree

Erik McCree is an American professional basketball player who last played for the BCM Gravelines-Dunkerque of the French LNB Pro A. He played college basketball for Louisiana Tech. McCree had been a part of Murray State University under his freshman year of college before transferring to Louisiana Tech University in 2013. In his junior year of college, he made it to the All-Conference USA Second Team, while he would improve to the All-Conference USA First Team in his senior year. After finishing his last four years of college from 2014–2017, he would end up being undrafted in the 2017 NBA draft. McCree would end up being a part of the Houston Rockets's Summer League squad for the 2017 NBA Summer League signing with the Miami Heat for their training camp squad, he would be waived from the Heat before the regular season began but would be assigned to the Sioux Falls Skyforce afterward. He would continue playing for them until December 2017, one day after his 24th birthday. On December 21, 2017, McGree signed a two-way contract with the Utah Jazz, replacing Eric Griffin's spot on the team.

Throughout the rest of the season, McCree would end up splitting his playing time between the Jazz and their NBA G League affiliate, the Salt Lake City Stars. McCree would make his official NBA debut on February 5, 2018, playing at around 2 minutes in a 133–109 blowout win over the New Orleans Pelicans. On August 1, 2018, McCree signed a deal with the Italian club VL Pesaro. On July 26, 2019, he has signed with BCM Gravelines-Dunkerque of the French LNB Pro A. Murray State bio Louisiana Tech bio

Second Battle of Simtokha Dzong

The Second Tibetan Invasion of Bhutan or the Second Battle of Simtokha Dzong was a military confrontation in 1634 between the supporters of Zhabdrung Ngawang Namgyal and the forces of the Tibetan Tsangpa dynasty and several Bhutanese lamas allied against him. The latter conquered Zhabdrung's seat, Simtokha Dzong, threatening to eliminate his young dominion; the castle's ammunition stores were accidentally ignited during the battle, resulting in an explosion that destroyed Simtokha Dzong and much of the Tibetan army. Seizing this chance, Zhabdrung's followers rallied and ousted the Tibetans from their territory, turning the battle into a decisive strategic victory of Ngawang Namgyal, paving the way for the Unification of Bhutan under his rule; as result of a theological and political dispute in Tibet over the reincarnation of the Drukpa Lineage-holder in the early 17th century, there were two men who claimed to be the rightful 18th abbot of Ralung Monastery: Ngawang Namgyal and Gyalwang Pagsam Wangpo.

Both were backed by different groups within the Drukpa sect, but the latter enjoyed the support of the Tsangpa dynasty that dominated central Tibet at the time. The conflict escalated, Ngawang Namgyal, whose "high level of intelligence and ambition were perceived as significant threats to the establishment", chose to go into voluntary exile in 1616/17. Invited by the lama of Gasa, he and his retinue settled down in the region that would become western Bhutan; this area was at the time divided among several different chiefdoms, petty kingdoms and Buddhist sects that fought for supremacy. Ngawang Namgyal was well received by the local Drukpa Kagyu clergy and began to garner support among the local populace while continuing to defy his rivals in Tibet, including the Tsangpa dynasty; as his fame and popularity grew, "he acquired the sobriquetZhabdrung Rinpoche’ ‘the precious jewel at whose feet one submits’."His growing influence angered not only his Tibetan enemies, but several powerful rival Buddhist schools of western Bhutan such as the Lhapa sect.

The Tsangpa invaded Bhutan in collaboration with the Lhapa hierarchs in order to eliminate Ngawang Namgyal in 1619. This first invasion was defeated by the Bhutanese chieftains who had rallied to Zhabdrung's support, but the conflict between him and the Tsangpa continued since a smallpox epidemic that killed King Karma Phuntsok Namgyal and many other members of the Tibetan dynasty was attributed to Ngawang Namgyal's alleged magical powers. After a time of self-chosen seclusion, Zhabdrung proclaimed his intention to become the spiritual and temporal ruler of Bhutan. While the Drukpa Kagyu and Nyingma schools as well as many chieftains accepted his political domination, several other lamas did not and continued to resist him; as he consolidated his control over the western valleys, Zhabdrung began to construct a strategically placed dzong as his seat of power in 1629. It was during the construction that five lamaist factions attacked his supporters; the following First Battle of Simtokha Dzong resulted in a decisive victory of Ngawang Namgyal's forces, while the dzong was finished in 1631.

As their own efforts to remove Zhabdrung failed, the lamas appealed for the new Tsangpa ruler, Karma Tenkyong, to launch another invasion of Bhutan to overthrow their rival. For some years, negotiations between the Tibetans and Ngawang Namgyal's followers took place to avoid war; these proved unfruitful, as result the Tsangpa launched their second invasion in 1634. This led the Bhutanese to claim that the Tibetan ruler had never wanted peace and that the negotiations had been a mere stragetic ploy. King Karma Tenkyong's exact motives for the invasion remain unclear, with Karma Phuntsho speculating that he either wanted to capture Zhabdrung, destabilize his rule in Bhutan, or obtain revered Tibetan relics that were in Bhutanese possession. In any case, the new invasion appears to have been much larger and better prepared than the one of 1619. Supported by the Bhutanese lamaist militias, King Karma Tenkyong sent five Tibetan divisions down into Bhutan: Four of these led an assault via the Paro and Gasa valleys, targeting Simtokha Dzong, while the fifth occupied the Bumthang valley, although that valley was home to the neutral Kingdom of Bumthang.

Why the Tsangpa dynasty occupied Bumthang is unknown, but John A. Ardussi speculates that either Ngawang Namgyal had a large following there or that the Tibetans at least believed it to be so. Once again, Zhabdrung's supporters rallied to his defense, but it became apparent that the Tibetan-Lamaist coalition was far stronger. Deputing his political and military duties to his confidant Tenzin Drukgyal, Ngawang Namgyal retreated to Jarogang in Khothangkha, resolved to escape to India if his domain would be destroyed; as the situation for Zhabdrung's forces became dire, Tibetan troops attacked Simtokha Dzong. Equipped with large numbers of matchlocks as well as Chinese trebuchets, Karma Tenkyong's troops stormed the palace; the Tibetans went on to demand hostages from Ngawang Namgyal. While Tibetan soldiers were looting the dzong, its gunpowder stores were ignited by accident. Simtokha Dzong, constructed just three years prior, killing most of the Tibetan forces present; as the Tibetans had been unaware of the ammunition, with the explosion coming out of nowhere, panic spread among the survivors.

Recognizing the chance, Zhabdrung's forces managed to overwhelm the remaining Tibetans in a counterattack. Bhutanese sources claim that "the few who survived, returned to Tibet with the news of a terrible defeat." Ardussi argues, that "