Sainte-Barbe is a municipality of Quebec, located within Le Haut-Saint-Laurent Regional County Municipality in the Montérégie administrative region. The population as of the Canada 2021 Census was 1,609.

Route 132 is the longest highway in Quebec. It follows the south shore of the Saint Lawrence River from the border with the state of New York in the hamlet of Dundee, west of Montreal to the Gulf of Saint Lawrence and circles the Gaspé Peninsula. This highway is known as the Navigator's Route. It passes through the Montérégie, Centre-du-Québec, Chaudière-Appalaches, Bas-Saint-Laurent and Gaspésie regions of the province.