Sakashita-juku was the forty-eighth of the fifty-three stations (shukuba) of the Tōkaidō connecting Edo with Kyoto in Edo period Japan. It was located in former Ise Province in what is now the Sakashita neighborhood of the city of Kameyama, Mie Prefecture, Japan.

The 53 Stations of the Tōkaidō are the rest areas along the Tōkaidō, which was a coastal route that ran from Nihonbashi in Edo to Sanjō Ōhashi in Kyoto. There were originally 53 government post stations along the Tōkaidō, where travelers had to present traveling permits at each station if wanting to cross.