Samson Agonistes is a tragic closet drama by John Milton. It appeared with the publication of Milton's Paradise Regained in 1671, as the title page of that volume states: "Paradise Regained / A Poem / In IV Books / To Which Is Added / Samson Agonistes". It is generally thought that Samson Agonistes was begun around the same time as Paradise Regained but was completed after the larger work, possibly very close to the date of publishing, but there is no certainty.

Samson was the last of the judges of the ancient Israelites mentioned in the Book of Judges and one of the last leaders who "judged" Israel before the institution of the monarchy. He is sometimes considered as an Israelite version of the popular Near Eastern folk hero also embodied by the Sumerian Gilgamesh and Enkidu, as well as the Greek Heracles. Samson was given superhuman powers by God in the form of extreme strength.