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Part of a radioactively labelled sequencing gel
Part of a radioactively labelled sequencing gel
DNA fragments are labelled with a radioactive or fluorescent tag on the primer (1), in the new DNA strand with a labeled dNTP, or with a labeled ddNTP
DNA fragments are labelled with a radioactive or fluorescent tag on the primer (1), in the new DNA strand with a labeled dNTP, or with a labeled ddNTP.
Sequence ladder by radioactive sequencing compared to fluorescent peaks
Sequence ladder by radioactive sequencing compared to fluorescent peaks
Capillary electrophoresis
Capillary electrophoresis
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An example of the results of automated chain-termination DNA sequencing.
An example of the results of automated chain-termination DNA sequencing.
Frederick Sanger, a pioneer of sequencing. Sanger is one of the few scientists who was awarded two Nobel prizes, one for the sequencing of proteins, a
Frederick Sanger, a pioneer of sequencing. Sanger is one of the few scientists who was awarded two Nobel prizes, one for the sequencing of proteins, and the other for the sequencing of DNA.
History of sequencing technology
History of sequencing technology
Genomic DNA is fragmented into random pieces and cloned as a bacterial library. DNA from individual bacterial clones is sequenced and the sequence is
Genomic DNA is fragmented into random pieces and cloned as a bacterial library. DNA from individual bacterial clones is sequenced and the sequence is assembled by using overlapping DNA regions.