The Santa Fe College Teaching Zoo is a 10-acre (4.0 ha) zoo in Gainesville, Florida. Located on Santa Fe College's main campus, it is the only college zookeeper training facility in the United States. Since 2000, the zoo has been accredited by the Association of Zoos and Aquariums (AZA).

Santa Fe College is a public college based in Gainesville, Florida, with satellite campuses in Alachua and Bradford counties. It is part of the Florida College System. It was established in 1965 as Santa Fe Junior College by the Florida Legislature and began offering classes in September 1966. As of Fall 2020, the school had an enrollment of 12,607 students, and offers associate and baccalaureate degree programs.