The Basilica of Santa Maria in Trastevere ; English: Our Lady in Trastevere) is a titular minor basilica in the Trastevere district of Rome, and one of the oldest churches of Rome. The basic floor plan and wall structure of the church date back to the 340s, and much of the structure to 1140–43. The first sanctuary was built in 221 and 227 by Pope Callixtus I and later completed by Pope Julius I. The church has large areas of important mosaics from the late 13th century by Pietro Cavallini.

The Nursing Madonna, Virgo Lactans, or Madonna Lactans, is an iconography of the Madonna and Child in which the Virgin Mary is shown breastfeeding the infant Jesus. In Italian it is called the Madonna del Latte. It was a common type in painting until the change in atmosphere after the Council of Trent, in which it was rather discouraged by the church, at least in public contexts, on grounds of propriety.