The Sedlec Ossuary is a Roman Catholic chapel, located beneath the Cemetery Church of All Saints, part of the former Sedlec Abbey in Sedlec, a suburb of Kutná Hora in the Czech Republic. The ossuary is estimated to contain the skeletons of between 40,000 and 70,000 people, whose bones have, in many cases, been artistically arranged to form decorations and furnishings for the chapel. The ossuary is among the most visited tourist attractions of the Czech Republic, drawing over 200,000 visitors annually.

Sedlec Abbey is a former Cistercian monastery in Sedlec, part of Kutná Hora in the Czech Republic. Founded in 1142, it was the first Cistercian foundation in Bohemia. Along with the rest of the Kutná Hora town centre, it was listed as a UNESCO World Heritage Site in 1995, because of its outstanding Baroque architecture. It is well known for housing the Sedlec Ossuary.