The Sharpness branch line is a railway in Gloucestershire, England, built by the Midland Railway (MR) to connect the port of Sharpness to the main Bristol and Gloucester Railway. The line opened for goods traffic in 1875 and to passenger traffic a year later. Passenger services were withdrawn in November 1964, but the line remains open for freight traffic to and from Sharpness Docks.

Sharpness is an English port in Gloucestershire, one of the most inland in Britain, and eighth largest in the South West England region. It is on the River Severn at grid reference SO669027, at a point where the tidal range, though less than at Avonmouth downstream, is still large.