The Sistine Chapel Choir, as it is generally called in English, or officially the Coro della Cappella Musicale Pontificia Sistina in Italian, is the Pope's personal choir. It performs at papal functions in the Sistine Chapel and in any other church in Rome where the Pope is officiating, including St. Peter's Basilica. One of the oldest choirs in the world, it was constituted as the Pope's personal choir by Pope Sixtus IV. Although it was established in the late 15th century, its roots go back to the 4th century and the reign of Pope Sylvester I.

The Sistine Chapel is a chapel in the Apostolic Palace, the pope's official residence in Vatican City. Originally known as the Cappella Magna, it takes its name from Pope Sixtus IV, who had it built between 1473 and 1481. Since that time, it has served as a place of both religious and functionary papal activity. Today, it is the site of the papal conclave, the process by which a new pope is selected. The chapel's fame lies mainly in the frescoes that decorate its interior, most particularly the Sistine Chapel ceiling and The Last Judgment, both by Michelangelo.