The United States has maintained an official presence in Slovenia since the early 1970s, when the United States Information Agency (USIS) opened a library and American press and cultural center in Ljubljana. From its opening through 1992, the American Center worked to develop closer grassroots relations between the United States and the people of the then-Socialist Republic of Slovenia, a constituent republic of the Socialist Federal Republic of Yugoslavia. On December 23, 1990, the Slovene people voted in a plebiscite to separate from greater Yugoslavia. On June 25, 1991, the new Republic of Slovenia officially declared its independence from the Federal Republic of Yugoslavia. A 10-day war commenced, during which Slovenian territorial troops fought off incursions by the Yugoslav People's Army. The United States formally recognized the new republic on April 7, 1992. To develop U.S. diplomatic relations with the new state, the United States opened a new Embassy in Ljubljana in August 1992. From the departure of Yousif Ghafari in January 2009 till November 2010, the U.S. Ambassador position was vacant. From November 2010 to 2015 it was held by Joseph A. Mussomeli. The Ambassador position is currently held by Jamie Harpootlian.

Slovene Americans or Slovenian Americans are Americans of full or partial Slovene or Slovenian ancestry. Slovenes mostly immigrated to America during the Slovene mass emigration period from the 1880s to World War I.