Sodium dodecyl sulfate
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Sodium dodecyl sulfate or sodium lauryl sulfate, sometimes written sodium laurilsulfate, is an organic compound with the formula CH311OSO3Na and structure H3C−11−O−S2−O−Na+. It is an anionic surfactan
Bottle of 20% sodium dodecyl sulfate in distilled water for use in the laboratory
SDS-PAGE
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SDS-PAGE is a discontinuous electrophoretic system developed by Ulrich K. Laemmli which is commonly used as a method to separate proteins with molecular masses between 5 and 250 kDa. The combined use
Sodium dodecyl sulfate
…that can degrade DNA, protecting the integrity of the isolated genetic material, and for denaturing proteins in preparation for electrophoresis in the SDS-PAGE technique. In the case of SDS-PAGE, the compound works by disrupting non-covalent bonds in the proteins, and so denaturing them, i.e. causing the protein…
Proteins of the erythrocyte membrane separated by SDS-PAGE according to their molecular masses
Sample combs with different numbers of pockets, each prong leaves a pocket in the gel when pulled out
Polymerised separating and stacking gel before removing the sample comb (white) between the spacers (black), in the stacking gel are small amounts of bromophenol blue for improved visibility, the separating gel is unstained
Electrophoresis chamber after a few minutes of electrophoresis. In the first pocket a size marker was applied with bromophenol blue, in the other pockets, the samples were added bromocresol green