A spot test in lichenology is a spot analysis used to help identify lichens. It is performed by placing a drop of a chemical reagent on different parts of the lichen and noting the colour change assoc
Spot tests on the foliose lichen Punctelia borreri showing thallus (top) and medulla (bottom). The pinkish-red colour change of the medulla in the C and KC tests indicate the presence of gyrophoric acid, a chemical feature that helps to distinguish it from similar species in the same genus.
Chemical spot tests on the crustose and saxicolous lichen Aspicilia epiglypta
UV-illuminated thallus and apothecia of the crustose lichen Ochrolechia africana; the yellowish colour results from the fluorescence of lichexanthone.
The Cladoniaceae are a family of lichen-forming fungi in the order Lecanorales, comprising about 560 species distributed amongst 18 genera. This family is one of the largest among lichen-forming fungi
Spot test (lichen)
…with secondary metabolites that have a free aldehyde group. This spot test was later shown to be particularly useful in the taxonomy of the family Cladoniaceae. Microcrystallization…
Cladonia subulata Flickr pellaea
The pixie cup, Cladonia pyxidata, was one of the first lichens to be scientifically described.
The t points to the leaf-like scales (squamules) of the ground-hugging primary thallus.
The upright secondary thallus, with stalks (podetia) topped by cup-shaped structures (apothecia). The red slime on the cup rims contains asexual reproductive spores (conidia).