The St. Johns and Lake Eustis Railway was a historic railroad in northern Central Florida. It ran from Astor on the St. Johns River south to Fort Mason and along Lake Eustis to Tavares and Lane Park. It also had track from Fort Mason to Leesburg, where it connected to the Florida Southern Railway.

Astor is an unincorporated community and census-designated place (CDP) in Lake County, Florida, United States, located on the west side of the St. Johns River between Lake George and Lake Dexter. The community straddles State Road 40, with the community of Volusia in Volusia County across the St. Johns to the east and Astor Park to the west. The population was 1,556 at the 2010 census. It is part of the Orlando–Kissimmee–Sanford Metropolitan Statistical Area.