St. Mark's Church in-the-Bowery is a parish of the Episcopal Church located at 131 East 10th Street, at the intersection of Stuyvesant Street and Second Avenue in the East Village neighborhood of Manhattan in New York City. The property has been the site of continuous Christian worship since the mid-17th century, making it New York City's oldest site of continuous religious practice. The structure is the second-oldest church building in Manhattan.

The East Village is a neighborhood on the East Side of Lower Manhattan in New York City, United States. It is roughly defined as the area east of the Bowery and Third Avenue, between 14th Street on the north and Houston Street on the south. The East Village contains three subsections: Alphabet City, in reference to the single-letter-named avenues that are located to the east of First Avenue; Little Ukraine, near Second Avenue and 6th and 7th Streets; and the Bowery, located around the street of the same name.