St Ambrose's Church is in the village of Grindleton, which is situated about 3 miles (5 km) northeast of Clitheroe, Lancashire, England. It is an active Anglican parish church in the deanery of Whalley, the archdeaconry of Blackburn, and the Diocese of Blackburn. Its benefice is united with that of St Peter and St Paul, Bolton by Bowland. The church is recorded in the National Heritage List for England as a designated Grade II listed building.

Grindleton is a village and civil parish in the Ribble Valley district of the English county of Lancashire, traditionally in the West Riding of Yorkshire. Its 3,700 acres sit within the Forest of Bowland. The population of the civil ward taken at the 2011 census was 772.