St Ambrose's Church is a Roman Catholic parish church in Heathgate Avenue, Speke, Liverpool, Merseyside, England. It is an active parish church in the Archdiocese of Liverpool and the Pastoral Area of Woolton and Halewood. The church is recorded in the National Heritage List for England as a designated Grade II listed building.

Speke is a suburb of Liverpool, Merseyside, England. It is 8 miles (12.9 km) southeast of the city centre. Located near the widest part of the River Mersey, it is bordered by the suburbs of Garston and Hunts Cross, and nearby to Halewood, Hale Village, and Widnes. The rural area of Oglet borders its south.