St Bertoline's Church is in the village of Barthomley, Cheshire, England. The church is recorded in the National Heritage List for England as a designated Grade I listed building. It is an active Anglican parish church in the diocese of Chester, the archdeaconry of Macclesfield and the deanery of Congleton.

Barthomley is a village and ancient parish, and is now a civil parish in the unitary authority of Cheshire East and the ceremonial county of Cheshire, England. According to the 2001 and the 2011 census' the parish had a population of 202. The village is situated near junction 16 of the M6 motorway and by the border with Staffordshire. It is about three miles south-west of Alsager.