The St John's Anglican Church, officially known as the Church of St. John the Evangelist, is a heritage-listed active Anglican church located at 120 Darlinghurst Road in the Sydney suburb of Darlinghurst, New South Wales, Australia. The church and its associated buildings were added to the New South Wales State Heritage Register on 2 April 1999. It was also listed on the former Register of the National Estate.

Darlinghurst is an inner-city suburb in the eastern suburbs of Sydney, New South Wales, Australia. Darlinghurst is located immediately east of the Sydney central business district (CBD) and Hyde Park, within the local government area of the City of Sydney. It is often colloquially referred to as "Darlo".