St Mary the Virgin is a parish church in the Church of England in Wirksworth, Derbyshire. It is a Grade I listed building. The existing building dates mostly from the 13th–15th centuries, but notable survivals from the Anglo-Saxon period indicate a church has stood on this site since at least the 8th century AD. It was restored in 1820, then in 1870 by Sir Gilbert Scott.

Wirksworth is a market, and former quarry town in the Derbyshire Dales district of Derbyshire, England. Its population of 4,904 in the 2021 census was estimated at 5,220 in 2023. Wirksworth contains the source of the River Ecclesbourne. The town was granted a market charter by Edward I in 1306 and still holds a market on Tuesdays in the Memorial Gardens. The parish church of St Mary's is thought to date from 653. The town developed as a centre for lead mining and stone quarrying. Many lead mines were owned by the Gell family of nearby Hopton Hall.