The Stainforth and Keadby Canal is a navigable canal in South Yorkshire and Lincolnshire, England. It connects the River Don Navigation at Bramwith to the River Trent at Keadby, by way of Stainforth, Thorne and Ealand, near Crowle. It opened in 1802, passed into the control of the River Don Navigation in 1849, and within a year was controlled by the first of several railway companies. It became part of the Sheffield and South Yorkshire Navigation, an attempt to remove several canals from railway control, in 1895. There were plans to upgrade it to take larger barges and to improve the port facilities at Keadby, but the completion of the New Junction Canal in 1905 made this unnecessary, as Goole could easily be reached and was already a thriving port.

The River Don Navigation was the result of early efforts to make the River Don in South Yorkshire, England, navigable between Fishlake and Sheffield. The Dutch engineer Cornelius Vermuyden had re-routed the mouth of the river in 1626, to improve drainage, and the new works included provision for navigation, but the scheme did not solve the problem of flooding, and the Dutch River was cut in 1635 to link the new channel to Goole. The first Act of Parliament to improve navigation on the river was obtained in 1726, by a group of Cutlers based in Sheffield; the Corporation of Doncaster obtained an Act in the following year for improvements to the lower river. Locks and lock cuts were built and by 1751 the river was navigable to Tinsley.