Staining
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Staining is a technique used to enhance contrast in samples, generally at the microscopic level. Stains and dyes are frequently used in histology, in cytology, and in the medical fields of histopathol
A stained histological specimen, sandwiched between a glass microscope slide.
Example of negative staining
Microscopic view of a histologic specimen of human lung tissue stained with hematoxylin and eosin.
PAS diastase showing the fungus Histoplasma.
H&E stain
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Hematoxylin and eosin stain is one of the principal tissue stains used in histology. It is the most widely used stain in medical diagnosis and is often the gold standard. For example, when a pathologi
Staining
…This stain is performed through the use of both red coloured carbol fuchsin that stains the bacteria and a counter stain such as methylene blue. Haematoxylin and eosin staining is frequently used in histology to examine thin tissue sections. Haematoxylin stains cell nuclei blue, while eosin stains cytoplasm, connective tissue…
Retina (part of the eye) stained with hematoxylin and eosin, cell nuclei stained blue-purple and extracellular material stained pink
Rack of slides being removed from a bath of hematoxylin stain
Ductal carcinoma in situ (DCIS) in breast tissue, cell nuclei (blue-purple), extracellular material (pink)