Stephens City is an incorporated town in the southern part of Frederick County, Virginia, United States, with a population of 2,016 at the time of the 2020 census, and an estimated population of 2,096 in 2022. Founded by Peter Stephens in the 1730s, the colonial town was chartered and named for Lewis Stephens in October 1758. It was originally settled by German Protestants from Heidelberg.

U.S. Route 340 is a spur route of US 40, and runs from Greenville, Virginia, to Frederick, Maryland. In Virginia, it runs north–south, parallel and east of US 11, from US 11 north of Greenville via Waynesboro, Grottoes, Elkton, Luray, Front Royal, and Berryville to the West Virginia state line. A short separate piece crosses northern Loudoun County on its way from West Virginia to Maryland.